Aug 2026· Journal of Food Protection· Vol 89, pp.
100900
· 0 citations· 27 references
Medicine
Abstract
Reused liquid batter systems in retail food-establishment represent a potential yet poorly studied source of seafood allergen cross-contact. This study quantified the accumulation of shrimp and cod proteins in a liquid batter across 20 sequential preparation batches, each representing a single portion (20-25 g), and evaluated their transfer to subsequently prepared chicken, mushroom, and zucchini, both before and after deep frying at 180°C. Allergen concentrations were measured using four commercial ELISA kits (two kits per species). Shrimp protein concentrations increased about 7-fold between batches 5 and 20, while cod protein concentrations increased approximately 3-fold over the same interval. Overall, cod residues accumulated in the batter at substantially higher levels than shrimp across all batches. Shrimp and cod proteins were detected in all three non-seafood items following preparation in the reused batter. Frying reduced detectable allergen levels by 1-67% for shrimp and 74-88% for cod. Consumer exposure estimates for shrimp did not exceed the 200 mg reference dose in any food type before or after frying, whereas cod exposure exceeded the 5 mg fish-protein reference dose even at the 25th percentile of consumption across all food types. These findings demonstrate that seafood allergens accumulate and transfer readily in reused liquid batter, posing a potential allergic risk in cross-contacted products even after frying.
Microplastics (MPs) are ubiquitous contaminants in the marine environment. The potential pathway through the food web to humans is a general public concern. Commonly consumed marine organisms have never been studied for the presence of MPs as an entry route into the food chain. This study selected
Penaeus monodon
(tiger prawn) and
Metapenaeus ensis
(greasyback shrimp) because of their commercial importance, high consumption rates, and consistent availability in Miri, Sarawak, Malaysia. Samples were collected from major fish markets supplied by both coastal fisheries and aquaculture farms. The gastrointestinal tracts of both species were analyzed under a microscope, and Attenuated Total Reflectance Fourier-Transform Infrared (ATR–FTIR) spectroscopy was performed on suspected MPs based on their physical characteristics. All samples contained plastic: 280 ± 91 pieces/kg wet weight for
P. monodon
and 457 ± 171 pieces/kg for
M. ensis
. Fibers were the dominant shape of the MPs observed, followed by fragments and pellets; black was also the dominant color. Most of the particles determined by size were within 500 to 1000 μm in dimension. Polyester and polystyrene were the two major types of polymers by the FTIR analysis. This calls for urgent reinforcement in food safety measures accompanied by environmental policy interventions directed towards microplastic contamination in seafood that encapsulates potential health risk avenues to consumers as well as broader ecological impacts.
W. Wong, Tony Hadibarata, R. A. Kristanti et al.· Environmental Sustainability· 0 citations
Smoked fish is widely consumed in Nigeria and may serve as a route of exposure to microbial contamination and toxic metals. This study assessed microbial quality, spoilage indicators, proximate composition, and heavy metal content associated with smoked Clarias gariepinus obtained from two farms (A and B) and two markets (A and B) in Ibadan, Nigeria, with implications for public health. Spoilage indicators were higher in market-sourced samples, with peroxide values ranging from 0.93 ± 0.40 mEq/kg in Farm B to 3.37 ± 0.29 mEq/kg in Market B. Total volatile base nitrogen and thiobarbituric acid values were also elevated, indicating increased lipid oxidation and protein degradation. Total viable (3.68 ± 0.79) × 10⁴ and fungal counts (2.66 ± 0.54) × 10⁵ were higher in market samples, although coliforms were not detected. Microbial profiling revealed greater diversity in market fish, including Pseudomonas, Aspergillus, Saccharomyces, and Geotrichum. Proximate composition was comparable across sources, with low moisture content (4.70–6.70%) and water activity (0.850–0.910), reflecting effective drying. Heavy metal concentrations were generally low in farmed fish but higher in market samples, particularly Market B, following the order arsenic > chromium > lead > cadmium, while mercury was not detected. Estimated daily intake and target hazard quotient values were below established safety thresholds, indicating no health risk. However, elevated spoilage indices and metal levels in market fish highlight the need for routine monitoring to protect consumer health.
Keywords: Biochemical Spoilage; Fish; Markets; Microbial Quality
O. Olowojuni, Nana Kadidia Traore, F. Olaifa· Acta Aquatica· 0 citations
Simple Summary Fish farming must increase production while reducing reliance on fishmeal, fish oil and antibiotics. Edible fungi, such as oyster, shiitake and reishi mushrooms, contain bioactive compounds that may support intestinal function, feed utilization, and innate immune responses in fish. This review examines how edible fungi and fungal-derived compounds, especially β-glucans and chitin, may contribute to gut microbiota modulation and immune stimulation in diets for commercially important fish. The available evidence indicates that their biological effects depend strongly on the fish species, ingredient form, dietary inclusion level, processing method and experimental conditions. Omnivorous fish such as tilapia generally tolerate broader inclusion levels, whereas carnivorous salmonids appear to respond more consistently to low dietary levels of extracts or processed fractions. Therefore, edible fungi should be regarded as complementary functional ingredients rather than simple protein replacements, requiring species-specific formulation strategies, standardized ingredient characterization, and validation under commercial farming conditions.
Marco Valdés, J. Quiñones, Matías Cortés et al.· Animals· 0 citations
Chemical contamination of milk represents a relevant food safety concern, particularly in countries where systematic monitoring programs are still limited. In Algeria, information on human exposure to aflatoxin M1 (AFM1) and per- and polyfluoroalkyl substance (PFAS) contamination remains fragmented or lacking. This study assessed the occurrence of AFM1 and 17 PFAS in cow and goat milk and explored farm-level management practices potentially influencing contamination. Bulk-tank milk samples were collected from 26 dairy cattle and 11 dairy goat farms. Farm characteristics, including feeding strategies, water sources, grazing practices, and herd size, were recorded using structured questionnaires to characterize farm-level practices and explore their potential association with contamination patterns. AFM1 was detected in all samples. In cow milk, 96.1% exceeded the EU limit (0.05 µg/kg), with concentrations ranging from 0.048 to 0.410 µg/kg (mean: 0.102 µg/kg), although none exceeded the Codex Alimentarius limit (0.50 µg/kg). Goat milk showed a lower prevalence of samples above the EU limit (36.4%), but higher concentrations were observed (range 0.030–0.578 µg/kg; mean 0.193 µg/kg), with two samples (18.2%) exceeding the Codex limit. PFAS contamination was generally low: 73.0% of cow and 64.0% of goat samples were below detection limits, with only PFBS (cow milk) and PFDA (goat milk) quantified above LOQ. Because of the limited number of farms and the single sampling period, no robust associations could be established between farm-level variables and contaminant concentrations. This preliminary study provides an exploratory overview of AFM1 and PFAS occurrence in bovine and goat bulk milk from the sampled Algerian farms. These findings reveal widespread AFM1 contamination within the investigated farms, consistent with possible feed-borne AFB1 exposure, whereas PFAS occurrence appeared sporadic and generally low.
Maria Belkacemi, N. Fedala, Teresa Gazzotti et al.· Dairy· 0 citations
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