protein folding

Heat-Shock and Cold-Shock Proteins in Energy-Based Aesthetic Medicine.

Multimodal Co-Generation With Continuous Bitstream Diffusion

We show that continuous bitstream diffusion (CoBit), originally developed for language modeling, provides a unified framework for joint and conditional multimodal generation using the same training and sampling protocol. Across image-text, speech-text and protein sequence-structure modeling, models are trained separate...

zPocket: Better Pocket Finding with Frozen Co-Folding Features

Identifying protein-ligand binding sites (pockets) is important for understanding protein function and developing drugs. However, no public method has leveraged co-folding models for novel pocket prediction; furthermore, this task lacks a suitable benchmark as existing benchmarks are within the training distribution of...

TGF‐β1 Induced Methylation to Regulate SOCS3 Expression in Abnormal Activation of Vocal Fold Fibroblasts

TGF‐β1 may downregulate SOCS3 expression via DNMT1‐mediated promoter DNA methylation, and this process is speculated to participate in abnormal VFFs activation by relieving the inhibitory effect of SOCS3 on the JAK2/STAT3 signaling pathway.

Genes controlling polysaccharide synthesis, protein folding and basic metabolism ensure intra-host cell adaptation and replication of Yersinia enterocolitica

Pathogenesis of yersiniosis comprises cell-invasive events in which the bacteria subvert the endocytic-autophagolysosomal pathway of infected host cells to create an intracellular niche for bacterial replication. To determine potential virulence factors that enable intracellular proliferation of Yersinia enterocolitica...

A High-Throughput Programmatic Pipeline for Computational Reclassification of Variants of Uncertain Significance in LDLR: Structural Evidence for Disruption of the LDL-Receptor Class A Ligand-Binding Repeats

Familial hypercholesterolemia (FH) is a common autosomal-dominant disorder of lipoprotein metabolism, most frequently caused by loss-of-function variants in the low-density lipoprotein receptor gene (LDLR). Clinical genetic testing for FH is increasingly limited not by sequencing throughput but by interpretation throug...

EL-RAKHAWI DOCTRINE OF UNIVERSAL ABO BLOOD CONVERSION AND DECENTRALIZED BIOLOGICAL RESILIENCE ENZYMATIC PROTOCOL FOR CONVERSION OF BLOOD GROUPS A, B, AND AB TO TYPE O USING ADVANCED GLYCOSIDASE ENGINEERING AND MEMBRANE ENZYME REACTORS

EL-RAKHAWI DOCTRINE OF UNIVERSAL ABO BLOOD CONVERSION: SUMMARY This groundbreaking technical monograph presents a scientifically rigorous protocol for converting blood groups A, B, and AB to type O through advanced enzymatic engineering, addressing critical errors in previous proposals while establishing comprehensive...

Biological trajectories toward universal computation: Transposons and chaperone-assisted folding as models of molecular logic.

The question of whether biological systems implement computation-like processes is central to synthetic biology and theoretical biophysics. This paper proposes a formal conceptual framework in which two well-characterized molecular mechanisms-transposable elements ("jumping genes") and chaperone-mediated protein foldin...

Positive selection and structural disruption of KCNA5 by mutation in colorectal cancer

Computational results highlight that KCNA5 is under mutational selection in colorectal adenocarcinoma, and that these mutations are likely functional and induce phenotypic effects on tumors.

Advancing O-Glycosylation Analysis of Fc-Fusion Proteins at the Domain Level by HILIC-MS with Supercharging.

Fragment crystallizable (Fc)-fusion proteins are formed by the fusion of functional peptides or receptor extracellular domains (ECDs) with Fc. They are normally very heterogeneous with multiple N- and O-glycosylation sites and a high level of sialylation. Characterization of O-glycosylation in Fc-fusion proteins is imp...

Split-intein-mediated engineering of VSV-G-free extracellular vesicles for enhanced cargo loading and delivery of functional proteins

Extracellular Vesicles (EVs) are a heterogeneous group of membranous nanoparticles. Due to their unique properties, they are considered promising nanocarriers for therapeutic applications. Cargo loading and functional delivery to the target cell are challenging areas and are receiving increasing attention in EV resea...

Gain-of-function mutations in the RodZ juxtamembrane domain promote Rod system activity in Escherichia coli

The peptidoglycan (PG) cell wall protects bacteria from osmotic lysis and determines their shape. In rod-shaped bacteria, the lateral wall is elongated by the Rod system (elongasome). This synthetic machine is built around the RodA-PBP2 PG synthase complex, the actin-like protein MreB, and the accessory proteins MreC,...